At the same time, water buffalo type were detected with 14 individuals in Chinese buffaloes. 同时在中国水牛群体中检测到江河型水牛14个个体,其为中国水牛与江河型水牛的杂交后代。
In order to optimize the culture system of buffalo spermatogonial stem-like cells, isolation methods and factors affecting the proliferation of buffalo type A spermatogonia in vitro were investigated in this study. 为优化水牛类精原干细胞的分离、培养体系,本研究对水牛A型精原细胞分离纯化方法,体外增殖的影响因素及其生物学特性进行了探讨。
The Sertoli cells expressed special protein vimentin, could be used as feed layer. ( 3) In vitro culture characterizations of buffalo type A spermatogonia and spermatogonial stem-like cells were examined. 其中支持细胞表达其特异性波形蛋白,可用饲养层制作。(3)对水牛A型精原细胞体外培养特征及类精原干细胞特性进行了研究。